Meyer, Sybille G. E. and Wendt, Agnieszka E. and Scherer, Max and Liebisch, Gerhard and Kerkweg, Uta and Schmitz, Gerd and de Groot, Herbert (2012) Myriocin, an inhibitor of serine palmitoyl transferase, impairs the uptake of transferrin and low-density lipoprotein in mammalian cells. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 526 (1). pp. 60-68. ISSN 0003-9861,
Full text not available from this repository. (Request a copy)Abstract
The role of sphingolipids in clathrin-mediated endocytosis is only poorly understood in mammalian cells. Thus the relationship between sphingolipid de novo synthesis and clathrin-mediated endocytosis of transferrin were studied in L929 fibroblasts and two other cell lines. Endocytosis was measured using live cell imaging with fluorescent transferrin or I-125-transferrin. Lipids were primarily measured using electrospray ionization tandem mass spectrometry. At physiological temperature, transferrin uptake was significantly decreased by the inhibitor of serine palmitoyl transferase myriocin. Myriocin inhibited also the uptake of low-density lipoproteins. The endocytosis inhibition by myriocin could be released by the addition of sphingoid base and by the protein phosphorylation effectors phorbol-12-myristate, 13-acetate (PMA) and okadaic acid. Myriocin influenced not only sphingolipids but also the glycerophospholipid profile. The study of phosphatidylcholine species shows adaptations to more saturated, alkylated and longer fatty acid moieties. The reported results imply that in mammalian cells, at 37 degrees C, sphingolipid de novo synthesis is required for clathrin-mediated endocytosis. (C) 2012 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; CLATHRIN-MEDIATED ENDOCYTOSIS; SACCHAROMYCES-CEREVISIAE; PROTEIN-KINASE; TYROSINE PHOSPHORYLATION; LYSOPHOSPHATIDIC ACID; PHOSPHOLIPASE A(2); SPHINGOLIPIDS; CHOLESTEROL; Sphingolipids; Clathrin-mediated endocytosis; Transferrin; Low-density lipoprotein; Myriocin; Lipid profiling |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 May 2020 11:36 |
| Last Modified: | 05 May 2020 11:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18040 |
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