Yu, Xiong and Goforth, Charles and Meyer, Carolin and Rachel, Reinhard and Wirth, Reinhard and Schroeder, Gunnar F. and Egelman, Edward H. (2012) Filaments from Ignicoccus hospitalis Show Diversity of Packing in Proteins Containing N-Terminal Type IV Pilin Helices. JOURNAL OF MOLECULAR BIOLOGY, 422 (2). pp. 274-281. ISSN 0022-2836,
Full text not available from this repository. (Request a copy)Abstract
Bacterial motility is driven by the rotation of flagellar filaments that supercoil. The supercoiling involves the switching of coiled-coil protofilaments between two different states. In archaea, the flagellar filaments responsible for motility are formed by proteins with distinct homology in their N-terminal portion to bacterial Type IV pilins. The bacterial pilins have a single N-terminal hydrophobic alpha-helix, not the coiled coil found in flagellin. We have used electron cryo-microscopy to study the adhesion filaments from the archaeon Ignicoccus hospitalis. While I. hospitalis is non-motile, these filaments make transitions between rigid stretches and curved regions and appear morphologically similar to true archaeal flagellar filaments. A resolution of similar to 7.5 angstrom allows us to unambiguously build a model for the packing of these N-terminal alpha-helices, and this packing is different from several bacterial Type IV pill whose structure has been analyzed by electron microscopy and modeling. Our results show that the mechanism responsible for the supercoiling of bacterial flagellar filaments cannot apply to archaeal filaments. (C) 2012 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TYPHIMURIUM FLAGELLAR FILAMENTS; PILUS-STRUCTURE; ELECTRON CRYOMICROSCOPY; SALMONELLA FLAGELLA; BACTERIAL FLAGELLA; II SECRETION; RECONSTRUCTION; RESOLUTION; MODEL; POLYMORPHISM; electron microscopy; helical polymers; convergent evolution; archaea |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Wirth Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 May 2020 05:42 |
| Last Modified: | 06 May 2020 05:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18110 |
Actions (login required)
 |
View Item |
