Schreiber, Andrea and Shulhevich, Yury and Geraci, Stefania and Hesser, Juergen and Stsepankou, Dzmitry and Neudecker, Sabine and Koenig, Stefan and Heinrich, Ralf and Hoecklin, Friederike and Pill, Johannes and Friedemann, Jochen and Schweda, Frank and Gretz, Norbert and Schock-Kusch, Daniel (2012) Transcutaneous measurement of renal function in conscious mice. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 303 (5). F783-F788. ISSN 1931-857X,
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Schreiber A, Shulhevich Y, Geraci S, Hesser J, Stsepankou D, Neudecker S, Koenig S, Heinrich R, Hoecklin F, Pill J, Friedemann J, Schweda F, Gretz N, Schock-Kusch D. Transcutaneous measurement of renal function in conscious mice. Am J Physiol Renal Physiol 303: F783-F788, 2012. First published June 13, 2012; doi: 10.1152/ajprenal.00279.2012.-Determination of glomerular filtration rate (GFR) in conscious mice is cumbersome for the experimenter and stressful for the animals. Here we report on a simple new technique allowing the transcutaneous measurement of GFR in conscious mice. This approach extends our previously developed technique for rats to mice. The technique relies on a miniaturized device equipped with an internal memory that permits the transcutaneous measurement of the elimination kinetics of the fluorescent renal marker FITC-sinistrin. This device is described and validated compared with FITC-sinistrin plasma clearance in healthy, unilaterally nephrectomized and pcy mice. In summary, we describe a technique allowing the measurement of renal function in freely moving mice independent of blood or urine sampling as well as of laboratory assays.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLOMERULAR-FILTRATION-RATE; FLUORESCEIN-LABELED SINISTRIN; FITC-SINISTRIN; MARKER; RATS; CLEARANCE; GFR; fluorescein isothiocyanate-sinistrin; kidney function; glomerular filtration rate; transcutaneous; fluorometry |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Frank Schweda |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 May 2020 08:32 |
| Last Modified: | 06 May 2020 08:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18190 |
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