Treeck, Oliver and Belgutay, Derya and Haering, Julia and Schueler, Susanne and Lattrich, Claus and Ortmann, Olaf (2012) Network analysis of icb-1 gene function in human breast cancer cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 113 (9). pp. 2979-2988. ISSN 0730-2312, 1097-4644
Full text not available from this repository. (Request a copy)Abstract
Icb-1 is a human gene previously described by our group to exert important functions in cancer cells of different origin. We now performed microarray-based gene expression profiling with subsequent network modeling to further elucidate the role of icb-1 in breast cancer cells. Analyzing the effect of icb-1 knockdown on the transcriptome of MCF-7 cells, we found 151 differentially expressed genes exhibiting more than twofold changes, 97 of which were up- and 54 downregulated. Most of the upregulated genes were cancer-related genes associated with poor prognosis, invasion and metastasis, building an oncogenic network of TNF target genes. On the other hand, network analysis identified the downregulated genes to be primarily involved in interferon signaling and cellular apoptosis. Confirming these network data, we observed that cells with reduced levels of icb-1 exhibited an impaired response to the apoptosis inducers tamoxifen, staurosporine, actinomycin, and camptothecin. The data of this study suggest that icb-1 might exert a tumor-suppressor function in breast cancer and that its loss might confer relative resistance of breast cancer cells to apoptotic drugs. J. Cell. Biochem. 113: 29792988, 2012. (C) 2012 Wiley Periodicals, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANS-RETINOIC ACID; NECROSIS-FACTOR-ALPHA; X-LINKED INHIBITOR; XAF1 EXPRESSION; EPITHELIAL MUCIN; COLON-CANCER; IN-VITRO; CARCINOEMBRYONIC ANTIGEN; HEPATOCELLULAR-CARCINOMA; INDUCED APOPTOSIS; DNA MICROARRAY ANALYSIS; icb-1 GENE; BREAST CANCER CELL; APOPTOSIS; GENE EXPRESSION; GENE NETWORK |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2020 05:21 |
| Last Modified: | 07 May 2020 05:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18267 |
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