Heller, Anne and Fischer, Lorenz H. and Wolfbeis, Otto S. and Goepferich, Achim (2012) Long time monitoring of the respiratory activity of isolated mitochondria. EXPERIMENTAL CELL RESEARCH, 318 (14). pp. 1667-1672. ISSN 0014-4827,
Full text not available from this repository. (Request a copy)Abstract
We have investigated the ability of optical oxygen sensors incorporated in a microplate to determine the respiratory activity of cell fractions. Different cell fractions were monitored, in particular to evaluate the long term functionality of isolated mitochondria. It is possible to continuously sense respiratory activity of isolated mitochondria over time. We found that they are functional for three hours but stop respiring at a critical limit of 20% air saturation in the system. Furthermore, inhibition and enhancement of respiratory activity were detected. In conclusion, oxygen sensors are a powerful tool to evaluate the functionality of isolated mitochondria. (C) 2012 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN MYOCARDIAL-CELLS; OXYGEN; CANCER; DRUG; DISEASE; ASSAYS; Isolated mitochondria; Respiratory activity; Optical oxygen sensor; High throughput screening |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2020 05:11 |
| Last Modified: | 11 May 2020 05:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18293 |
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