Fischer, Susanne and Bayersdorfer, Florian and Harant, Eva and Reng, Renate and Arndt, Stephanie and Bosserhoff, Anja-Katrin and Schneuwly, Stephan (2012) fussel (fuss) - A Negative Regulator of BMP Signaling in Drosophila melanogaster. PLOS ONE, 7 (8): e42349. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
The TGF-beta/BMP signaling cascades control a wide range of developmental and physiological functions in vertebrates and invertebrates. In Drosophila melanogaster, members of this pathway can be divided into a Bone Morphogenic Protein (BMP) and an Activin-beta (Act-beta) branch, where Decapentaplegic (Dpp), a member of the BMP family has been most intensively studied. They differ in ligands, receptors and transmitting proteins, but also share some components, such as the Co-Smad Medea (Med). The essential role of Med is to form a complex with one of the two activating Smads, mothers against decapentaplegic (Mad) or dSmad, and to translocate together to the nucleus where they can function as transcriptional regulators of downstream target genes. This signaling cascade underlies different mechanisms of negative regulation, which can be exerted by inhibitory Smads, such as daughters against decapentaplegic (dad), but also by the Ski-Sno family. In this work we identified and functionally analyzed a new member of the Ski/Sno-family, fussel (fuss), the Drosophila homolog of the human functional suppressing element 15 (fussel-15). fuss codes for two differentially spliced transcripts with a neuronal expression pattern. The proteins are characterized by a Ski-Sno and a SAND homology domain. Overexpression studies and genetic interaction experiments clearly reveal an interaction of fuss with members of the BMP pathway, leading to a strong repression of BMP-signaling. The protein interacts directly with Medea and seems to reprogram the Smad pathway through its influence upon the formation of functional Mad/Medea complexes. This leads amongst others to a repression of downstream target genes of the Dpp pathway, such as optomotor blind (omb). Taken together we could show that fuss exerts a pivotal role as an antagonist of BMP signaling in Drosophila melanogaster.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TGF-BETA SUPERFAMILY; TRANSCRIPTIONAL COREPRESSOR; TUMOR-SUPPRESSOR; WING DEVELOPMENT; FAMILY-MEMBERS; SMAD PROTEINS; CO-REPRESSOR; GENE; SKI; DPP; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie Biology, Preclinical Medicine > Institut für Zoologie Biology, Preclinical Medicine > Institut für Zoologie > Entwicklungsbiologie (Prof. Dr. Stephan Schneuwly) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2020 06:53 |
| Last Modified: | 07 May 2020 06:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18307 |
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