Zhao, Xin'Ai and Harashima, Hirofumi and Dissmeyer, Nico and Pusch, Stefan and Weimer, Annika K. and Bramsiepe, Jonathan and Bouyer, Daniel and Rademacher, Svenja and Nowack, Moritz K. and Novak, Bela and Sprunck, Stefanie and Schnittger, Arp (2012) A General G1/S-Phase Cell-Cycle Control Module in the Flowering Plant Arabidopsis thaliana. PLOS GENETICS, 8 (8): e1002847. ISSN 1553-7404,
Full text not available from this repository. (Request a copy)Abstract
The decision to replicate its DNA is of crucial importance for every cell and, in many organisms, is decisive for the progression through the entire cell cycle. A comparison of animals versus yeast has shown that, although most of the involved cell-cycle regulators are divergent in both clades, they fulfill a similar role and the overall network topology of G1/S regulation is highly conserved. Using germline development as a model system, we identified a regulatory cascade controlling entry into S phase in the flowering plant Arabidopsis thaliana, which, as a member of the Plantae supergroup, is phylogenetically only distantly related to Opisthokonts such as yeast and animals. This module comprises the Arabidopsis homologs of the animal transcription factor E2F, the plant homolog of the animal transcriptional repressor Retinoblastoma (Rb)-related 1 (RBR1), the plant-specific F-box protein F-BOX-LIKE 17 (FBL17), the plant specific cyclin-dependent kinase (CDK) inhibitors KRPs, as well as CDKA; 1, the plant homolog of the yeast and animal Cdc2+/Cdk1 kinases. Our data show that the principle of a double negative wiring of Rb proteins is highly conserved, likely representing a universal mechanism in eukaryotic cell-cycle control. However, this negative feedback of Rb proteins is differently implemented in plants as it is brought about through a quadruple negative regulation centered around the F-box protein FBL17 that mediates the degradation of CDK inhibitors but is itself directly repressed by Rb. Biomathematical simulations and subsequent experimental confirmation of computational predictions revealed that this regulatory circuit can give rise to hysteresis highlighting the here identified dosage sensitivity of CDK inhibitors in this network.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT KINASE INHIBITORS; INTRINSICALLY UNSTRUCTURED PROTEINS; QUANTITATIVE PCR DATA; GAMETOPHYTE DEVELOPMENT; RETINOBLASTOMA PROTEIN; POLLEN DEVELOPMENT; MALE GAMETOGENESIS; PROLIFERATION; DIVISION; EXPRESSION; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 May 2020 05:02 |
| Last Modified: | 08 May 2020 05:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18334 |
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