Birner, Christoph and Dietl, Alexander and Deutzmann, Rainer and Schroeder, Josef and Schmid, Peter and Jungbauer, Carsten and Resch, Markus and Endemann, Dierk and Stark, Klaus and Riegger, Guenter and Luchner, Andreas (2012) Proteomic Profiling Implies Mitochondrial Dysfunction in Tachycardia-Induced Heart Failure. JOURNAL OF CARDIAC FAILURE, 18 (8). pp. 660-673. ISSN 1071-9164,
Full text not available from this repository. (Request a copy)Abstract
Background/Objectives: Molecular mechanisms of congestive heart failure as reflected by alterations of protein expression patterns are still incompletely analyzed. We therefore investigated intraventricular (ie, left ventricular congestive heart failure [LV-CHF] vs. LV-control [CTRL], and right ventricular [RV]-CHF vs. RV-CTRL) and interventricular (ie, LV-CHF vs. RV-CHF, and LV-CTRL vs. RV-CTRL) protein expression differences in an animal model. Methods: The model of rapid ventricular pacing in rabbits was combined with a proteomic approach using 2-dimensional gel electrophoresis. Identification of proteins was done by matrix-assisted laser desorption/ionization-tandem mass spectrometry (MALDI-MS/MS). Results: Rapid ventricular pacing-induced heart failure was characterized by LV dilatation, dysfunction, and hypotension as well as by increased BNP gene expression. By comparing LV-CHF vs. LV-CTRL, proteins were found to be underexpressed at 3 crucial points of cellular energy metabolism. In RV-CHF vs. RV-CTRL, proteins belonging to respiratory chain complexes were underexpressed, but additionally a disturbance in the nitric oxide-generating enzymatic apparatus was seen. Regarding the interventricular analyses, a stronger expression of energetic pathways was accompanied by an underexpression of contractile and stress response proteins in failing left vs. right ventricles. Finally, significant protein expression differences were found in LV-CTRL vs. RV-CTRL reflecting a higher expression of contractile, stress response, and respiratory chain proteins in LV tissue. Conclusions: In tachycardia-induced heart failure, significant inter- and intraventricular protein expression patterns were found with a predominance of proteins, which are involved in cellular energy metabolism. (J Cardiac Fail 2012;18:660-673)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 2-DIMENSIONAL ELECTROPHORESIS; FAILING HEART; ANIMAL-MODELS; DISEASE; EXPRESSION; MYOSIN; BNP; CARDIOMYOPATHY; PROGRESSION; EVOLUTION; Heart failure; energetics; proteomics |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Medicine > Lehrstuhl für Pathologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2020 06:46 |
| Last Modified: | 07 May 2020 06:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18368 |
Actions (login required)
 |
View Item |
