Hochstatter, Julia and Hoelzel, Michael and Rohrmoser, Michaela and Schermelleh, Lothar and Leonhardt, Heinrich and Keough, Rebecca and Gonda, Thomas J. and Imhof, Axel and Eick, Dirk and Laengst, Gernot and Nemeth, Attila (2012) Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA. JOURNAL OF BIOLOGICAL CHEMISTRY, 287 (29). pp. 24365-24377. ISSN 0021-9258,
Full text not available from this repository. (Request a copy)Abstract
Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modulation of Mybbp1a protein levels results in defects in pre-rRNA processing within the cell. Therefore, the protein may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMERASE-I TRANSCRIPTION; FACTOR TIF-IA; NUCLEAR ANTIGEN 1; SACCHAROMYCES-CEREVISIAE; NUCLEOLAR PROTEIN; MOLECULAR-CLONING; GENE-EXPRESSION; DEPENDENT TRANSCRIPTION; YEAST; CHROMATIN; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2020 05:43 |
| Last Modified: | 11 May 2020 05:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18444 |
Actions (login required)
 |
View Item |
