Marton, Mihaela L. and Fastner, Astrid and Uebler, Susanne and Dresselhaus, Thomas (2012) Overcoming Hybridization Barriers by the Secretion of the Maize Pollen Tube Attractant ZmEA1 from Arabidopsis Ovules. CURRENT BIOLOGY, 22 (13). pp. 1194-1198. ISSN 0960-9822,
Full text not available from this repository. (Request a copy)Abstract
A major goal of plant reproduction research is to understand and overcome hybridization barriers so that the gene pool of crop plants can be increased and improved upon. After successful pollen germination on a receptive stigma, the nonmotile sperm cells of flowering plants are transported via the pollen tube (PT) to the egg apparatus for the achievement of double fertilization. The PT path is controlled by various hybridization mechanisms probably involving a larger number of species-specific molecular interactions [1, 2]. The egg-apparatus-secreted polymorphic peptides ZmEA1 in maize [3] and LURE1 and LURE2 in Torenia foumieri [4] as well as TcCRP1 in T. concolor [5] were shown to be required for micropylar PT guidance, the last step of the PT journey. We report here that ZmEA1 attracts maize PTs in vitro and arrests their growth at higher concentrations. Furthermore, it binds to the subapical region of maize PT tips in a species-preferential manner. To overcome hybridization barriers at the level of gametophytic PT guidance, we expressed ZmEA1 in Arabidopsis synergid cells. Secreted ZmEA1 enabled Arabidopsis ovules to guide maize PT in vitro in a species-preferential manner to the micropylar opening of the ovule. These results demonstrate that the egg-apparatus-controlled reproductive-isolation barrier of PT guidance can be overcome even between unrelated plant families.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYNERGID CELL; FEMALE GAMETOPHYTE; IN-VITRO; GUIDANCE; GROWTH; IDENTIFICATION; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2020 05:46 |
| Last Modified: | 11 May 2020 05:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18453 |
Actions (login required)
 |
View Item |
