Pasikowska, Monika and Palamarczyk, Grazyna and Lehle, Ludwig (2012) The essential endoplasmic reticulum chaperone Rot1 is required for protein N- and O-glycosylation in yeast. GLYCOBIOLOGY, 22 (7). pp. 939-947. ISSN 0959-6658,
Full text not available from this repository. (Request a copy)Abstract
Rot1 is an essential yeast protein originally shown to be implicated in such diverse processes such as beta-1,6-glucan synthesis, actin cytoskeleton dynamics or lysis of autophagic bodies. More recently also a role as a molecular chaperone has been discovered. Here, we report that Rot1 interacts in a synthetic manner with Ost3, one of the nine subunits of the oligosaccharyltransferase (OST) complex, the key enzyme of N-glycosylation. The deletion of OST3 in the rot1-1 mutant causes a temperature sensitive phenotype as well as sensitivity toward compounds interfering with cell wall biogenesis such as Calcofluor White, caffeine, Congo Red and hygromycin B, whereas the deletion of OST6, a functional homolog of OST3, has no effect. OST activity in vitro determined in membranes from rot1-1ost3 delta cells was found to be decreased to 45% compared with wild-type membranes, and model glycoproteins of N-glycosylation, like carboxypeptidase Y, Gas1 or dipeptidyl aminopeptidase B, displayed an underglycosylation pattern. By affinity chromatography, a physical interaction between Rot1 and Ost3 was demonstrated. Moreover, Rot1 was found to be involved also in the O-mannosylation process, as the glycosylation of distinct glycoproteins of this type were affected as well. Altogether, the data extend the role of Rot1 as a chaperone required to ensure proper glycosylation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FARNESYL DIPHOSPHATE SYNTHASE; SACCHAROMYCES-CEREVISIAE; OLIGOSACCHARYL TRANSFERASE; SECRETORY PATHWAY; BACTERIAL OLIGOSACCHARYLTRANSFERASE; LINKED GLYCOSYLATION; MOLECULAR CHAPERONE; MEMBRANE-PROTEIN; LEISHMANIA-MAJOR; GENOMIC ANALYSES; dolichol; N-glycosylation; O-glycosylation; oligosaccharyltransferase; ROT1; Saccharomyces cerevisiae |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 May 2020 11:36 |
| Last Modified: | 12 May 2020 11:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18555 |
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