Dittmar, Michael S. and Petermichl, Walter and Schlachetzki, Felix and Graf, Bernhard M. and Gruber, Michael (2012) Isoflurane Induces Endothelial Apoptosis of the Post-Hypoxic Blood-Brain Barrier in a Transdifferentiated Human Umbilical Vein Edothelial Cell Model. PLOS ONE, 7 (6): e38260. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Isoflurane is a popular volatile anesthetic agent used in humans as well as in experimental animal research. In previous animal studies of the blood-brain barrier (BBB), observations towards an increased permeability after exposure to isoflurane are reported. In this study we investigated the effect of a 2-hour isoflurane exposure on apoptosis of the cerebral endothelium following 24 hours of hypoxia in an in vitro BBB model using astrocyte-conditioned human umbilical vein endothelial cells (AC-HUVECs). Apoptosis of AC-HUVECs was investigated using light microscopy of the native culture for morphological changes, Western blot (WB) analysis of Bax and Bcl-2, and a TUNEL assay. Treatment of AC-HUVECs with isoflurane resulted in severe cellular morphological changes and a significant dose-dependent increase in DNA fragmentation, which was observed during the TUNEL assay analysis. WB analysis confirmed increases in pro-apoptotic Bax levels at 4 hours and 24 hours and decreases in anti-apoptotic Bcl-2 in a dose-dependent manner compared with the control group. These negative effects of isoflurane on the BBB after a hypoxic challenge need to be taken into account not only in experimental stroke research, but possibly also in clinical practice.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FOCAL CEREBRAL-ISCHEMIA; NEUROVASCULAR UNIT; ARTERY OCCLUSION; NITRIC-OXIDE; RATS; INJURY; STROKE; PERMEABILITY; INHIBITION; ANESTHESIA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 May 2020 05:42 |
| Last Modified: | 12 May 2020 05:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18571 |
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