Martinez Ferreras, Fernando and Wolfbeis, Otto S. and Gorris, Hans H. (2012) Dual lifetime referenced fluorometry for the determination of doxorubicin in urine. ANALYTICA CHIMICA ACTA, 729. pp. 62-66. ISSN 0003-2670, 1873-4324
Full text not available from this repository. (Request a copy)Abstract
Dual lifetime referencing (DLR) is introduced as a rapid and self-referenced method for measuring the concentration of a fluorescent analyte in solution. The fluorescent cancer chemotherapeutic doxorubicin was chosen as a medically relevant analyte and blended with a reference dye (Ru(dpp)(3)) that displays overlapping excitation and emission spectra. The relative contributions of the short-lived (nanoseconds) fluorescent analyte and the long-lived (microseconds) reference dye define the observed lifetime. Measuring this lifetime by both frequency-domain DLR and time-domain DLR yields similar analytical ranges and limits of detection (0.4 mu M). To assess the matrix effect of medical samples, the standard addition method was employed to both modes of DLR. Urine was spiked with doxorubicin and recovery rates of >= 97% were obtained. (C) 2012 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLUORESCENCE; ACRYLONITRILE; COMPLEXES; POLYMERIZATION; EMISSION; CANCER; PH; Doxorubicin; Fluorescence lifetime; Self-referenced measurements |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 May 2020 12:24 |
| Last Modified: | 11 May 2020 12:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18600 |
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