Ousingsawat, Jiraporn and Schreiber, Rainer and Kunzelmann, Karl (2012) Differential contribution of SLC26A9 to Cl- conductance in polarized and non-polarized epithelial cells. JOURNAL OF CELLULAR PHYSIOLOGY, 227 (6). pp. 2323-2329. ISSN 0021-9541,
Full text not available from this repository. (Request a copy)Abstract
SLC26 proteins function as anion exchangers and Cl- channels. SLC26A9 has been proposed to be a constitutively active and CFTR-regulated anion conductance in human bronchial epithelia. This positive interaction between two Cl- channels has been questioned by others and evidence has been provided that CFTR rather inhibits the transport activity of SLC26A9. We therefore examined the functional interaction between CFTR and SLC26A9 in polarized airway epithelial cells and in non-polarized HEK293 cells expressing CFTR and SLC26A9. We found that SLC26A9 provides a constitutively active basal Cl- conductance in polarized grown CFTR-expressing CFBE airway epithelial cells, but not in cells expressing F508del-CFTR. In polarized CFTR-expressing cells, SLC26A9 also contributes to both Ca2+- and CFTR-activated Cl- secretion. In contrast in non-polarized HEK293 cells co-expressing CFTR/SLC26A9, the baseline Cl- conductance provided by SLC26A9 was inhibited during activation of CFTR. SLC26A9 and CFTR behave differentially in polarized and non-polarized cells, which may explain earlier conflicting data. J. Cell. Physiol. 227: 23232329, 2012. (c) 2011 Wiley Periodicals, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYSTIC-FIBROSIS; CFTR ACTIVITY; SECRETION; ACTIVATION; TRANSPORT; FLUID; LINES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 May 2020 09:10 |
| Last Modified: | 13 May 2020 09:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18706 |
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