Kropinski, Andrew M. and Van den Bossche, An and Lavigne, Rob and Noben, Jean-Paul and Babinger, Patrick and Schmitt, Ruediger (2012) Genome and proteome analysis of 7-7-1, a flagellotropic phage infecting Agrobacterium sp H13-3. VIROLOGY JOURNAL, 9: 102. ISSN 1743-422X,
Full text not available from this repository. (Request a copy)Abstract
Background: The flagellotropic phage 7-7-1 infects motile cells of Agrobacterium sp H13-3 by attaching to and traveling along the rotating flagellar filament to the secondary receptor at the base, where it injects its DNA into the host cell. Here we describe the complete genomic sequence of 69,391 base pairs of this unusual bacteriophage. Methods: The sequence of the 7-7-1 genome was determined by pyro(454)sequencing to a coverage of 378-fold. It was annotated using MyRAST and a variety of internet resources. The structural proteome was analyzed by SDS-PAGE coupled electrospray ionization-tandem mass spectrometry (MS/MS). Results: Sequence annotation and a structural proteome analysis revealed 127 open reading frames, 84 of which are unique. In six cases 7-7-1 proteins showed sequence similarity to proteins from the virulent Burkholderia myovirus BcepB1A. Unique features of the 7-7-1 genome are the physical separation of the genes encoding the small (orf100) and large (orf112) subunits of the DNA packaging complex and the apparent lack of a holin-lysin cassette. Proteomic analysis revealed the presence of 24 structural proteins, five of which were identified as baseplate (orf7), putative tail fibre (orf102), portal (orf113), major capsid (orf115) and tail sheath (orf126) proteins. In the latter case, the N-terminus was removed during capsid maturation, probably by a putative prohead protease (orf114).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLAGELLA SPECIFIC BACTERIOPHAGE; BURKHOLDERIA-CEPACIA-COMPLEX; TRANSFER-RNA GENES; RHIZOBIUM-LUPINI; PSEUDOMONAS-AERUGINOSA; STRUCTURE PREDICTION; MASS-SPECTROMETRY; PHI-CTX; SEQUENCE; IDENTIFICATION; Agrobacterium; Phage evolution; Phage ecology; Genome; Proteome; Complex flagellum; Bioinformatics; Posttranslational modification |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 May 2020 09:12 |
| Last Modified: | 13 May 2020 09:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18707 |
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