Thomas, Anja and Stevens, Axel P. and Klein, Matthias S. and Hellerbrand, Claus and Dettmer, Katja and Gronwald, Wolfram and Oefner, Peter J. and Reinders, Joerg (2012) Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet. PROTEOMICS, 12 (9). pp. 1437-1451. ISSN 1615-9853, 1615-9861
Full text not available from this repository. (Request a copy)Abstract
Despite the increasing incidence of nonalcoholic steatohepatitis (NASH) with the rise in lifestyle-related diseases such as the metabolic syndrome, little is known about the changes in the liver proteome that precede the onset of inflammation and fibrosis. Here, we investigated early changes in the liver-soluble proteome of female C57BL/6N mice fed an NASH-inducing diet by 2D-DIGE and nano-HPLC-MS/MS. In parallel, histology and measurements of hepatic content of triglycerides, cholesterol and intermediates of the methionine cycle were performed. Hepatic steatosis manifested itself after 2 days of feeding, albeit significant changes in the liver-soluble proteome were not evident before day 10 in the absence of inflammatory or fibrotic signs. Proteomic alterations affected mainly energy and amino acid metabolism, detoxification processes, urea cycle, and the one-carbon/S-adenosylmethionine pathways. Additionally, intermediates of relevant affected pathways were quantified from liver tissue, confirming the findings from the proteomic analysis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AROMATICUM STRAIN EBN1; BACTERIUM RHODOPSEUDOMONAS-PALUSTRIS; DENITRIFYING BACTERIUM; ANAEROBIC DEGRADATION; BETA-OXIDATION; COENZYME-A; HYDROXYCINNAMOYL-COENZYME; GEL-ELECTROPHORESIS; LIGNIN BIOSYNTHESIS; THAUERA-AROMATICA; Animal proteomics; Liver proteome; Metabolomics; Nonalcoholic steatohepatitis; Two-dimensional differential in-gel electrophoresis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 May 2020 05:46 |
| Last Modified: | 15 May 2020 05:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18803 |
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