Moehrlen, Ueli and Lechner, Anja and Baeumel, Monika and Dostert, Karin and Roehrl, Johann and Meuli, Martin and Maennel, Daniela N. and Hamacher, Juerg (2012) Immune cell populations and cytokine production in spleen and mesenteric lymph nodes after laparoscopic surgery versus conventional laparotomy in mice. PEDIATRIC SURGERY INTERNATIONAL, 28 (5). pp. 507-513. ISSN 0179-0358,
Full text not available from this repository. (Request a copy)Abstract
There is evidence that open as well as minimally invasive abdominal surgery impair post-operative innate and acquired immune function. To compare the impact of these approaches as well as the one of different peritoneal gas exposures on immune function, we investigated cellular as well as cytokine-based immune parameters in mesenteric lymph nodes and the spleen postoperatively. Mice (n = 26) were randomly assigned to the 4 study groups: (1) sham controls undergoing anesthesia alone, (2) laparotomy, and (3) air, or (4) carbon dioxide pneumoperitoneum. Mice were sacrificed 48 h after the intervention, and their spleens and mesenteric lymph nodes were harvested. Cytokine production (TNF-alpha, IL-6, IL-10, and IFN-gamma), splenic T cell subpopulations (cytotoxic T cells, T helper cells, and regulatory T cells) were analyzed. TNF-alpha production of splenocytes 16 h after ex vivo lipopolysaccharides (LPS) stimulation was significantly increased in the laparotomy group compared to all other groups. In contrast, TNF-alpha production of lymph node cells and IL-6 production of splenocytes after ex vivo LPS stimulation did not differ significantly between the groups. The numbers of regulatory T cells (Treg) in the spleen differed between groups. A significant reduction in Treg cell frequency was detected in the CO2 insufflation group compared to the laparotomy and the air insufflation group. Our findings demonstrate a distinct difference in immune effector functions and cellular composition of the spleen with regard to splenic TNF-alpha production and increased numbers of Treg cells in the spleen. These findings are in line with a higher peritoneal inflammatory status consequent to peritoneal air rather than CO2 exposure. Treg turned out to be key modulators of postoperative dysfunction of acquired immunity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARBON-DIOXIDE; OPEN CHOLECYSTECTOMY; RANDOMIZED-TRIAL; MURINE MODEL; PNEUMOPERITONEUM; PRESERVATION; MACROPHAGES; APOPTOSIS; SEPSIS; INFLAMMATION; Laparoscopy; Laparotomy; Pneumoperitoneum; Immune function; Mouse model; Lymphocyte subsets; T-lymphocytes; Regulatory; Cytokines |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 May 2020 06:05 |
| Last Modified: | 15 May 2020 06:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18822 |
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