Schmidt, Sebastian and Juergenliemk, Guido and Skaltsa, Helen and Heilmann, Joerg (2012) Phloroglucinol derivatives from Hypericum empetrifolium with antiproliferative activity on endothelial cells. PHYTOCHEMISTRY, 77. pp. 218-225. ISSN 0031-9422,
Full text not available from this repository. (Request a copy)Abstract
Five acylphloroglucinols substituted with monoterpenoids (empetrifelixin A-D and empetrikajaforin), three known monocyclic acylphloroglucinols and one monocyclic acylphloroglucinol were isolated from a petrol ether extract of Hypericum empetrifolium after fractionation by flash chromatography on silica gel, RP-18 and subsequent purification by preparative HPLC (RP-18). Their structures were elucidated by 1D, 2D NMR techniques and HREIMS. To determine a possible anti-angiogenic activity, inhibition of cell proliferation was measured using a human microvascular endothelial cell line (HMEC-1). Subconfluent grown HMEC-1 cells were treated with all compounds isolated in sufficient amounts and stained with crystal violet. Highest activity was observed for empetrifelixin A and empetrifelixin D showing a concentration dependent inhibition of cell proliferation with IC50 values of 6.5 +/- 0.1 and 7.3 +/- 0.4 mu M, respectively. Empetrifelixin A also showed activity in a cell migration assay with HMEC-1 cells in low micromolar concentrations. (C) 2011 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACYLPHLOROGLUCINOL DERIVATIVES; HYPERFORIN; ANTIBACTERIAL; INHIBITOR; PAPUANUM; ERECTUM; Hypericum empetrifolium; Hypericaceae; Acylphloroglucinols; Anti-angiogenic activity; NMR guided fractionation |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 May 2020 07:16 |
| Last Modified: | 15 May 2020 07:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18839 |
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