Stemberger, Christian and Dreher, Stefan and Tschulik, Claudia and Piossek, Christine and Bet, Jeannette and Yamamoto, Tori N. and Schiemann, Matthias and Neuenhahn, Michael and Martin, Klaus and Schlapschy, Martin and Skerra, Arne and Schmidt, Thomas and Edinger, Matthias and Riddell, Stanley R. and Germeroth, Lothar and Busch, Dirk H. (2012) Novel Serial Positive Enrichment Technology Enables Clinical Multiparameter Cell Sorting. PLOS ONE, 7 (4): e35798. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
A general obstacle for clinical cell preparations is limited purity, which causes variability in the quality and potency of cell products and might be responsible for negative side effects due to unwanted contaminants. Highly pure populations can be obtained best using positive selection techniques. However, in many cases target cell populations need to be segregated from other cells by combinations of multiple markers, which is still difficult to achieve - especially for clinical cell products. Therefore, we have generated low-affinity antibody-derived Fab-fragments, which stain like parental antibodies when multimerized via Strep-tag and Strep-Tactin, but can subsequently be removed entirely from the target cell population. Such reagents can be generated for virtually any antigen and can be used for sequential positive enrichment steps via paramagnetic beads. First protocols for multiparameter enrichment of two clinically relevant cell populations, CD4(high)/CD25(high)/CD45RA(high) 'regulatory T cells' and CD8(high)/CD62L(high)/CD45RA(neg) 'central memory T cells', have been established to determine quality and efficacy parameters of this novel technology, which should have broad applicability for clinical cell sorting as well as basic research.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VERSUS-HOST-DISEASE; ONE-STEP PURIFICATION; REGULATORY T-CELLS; F-AB FRAGMENT; ADOPTIVE TRANSFER; CRYSTAL-STRUCTURE; ANTIBODY; LYMPHOCYTES; TRANSPLANTATION; IMMUNOTHERAPY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 May 2020 05:41 |
| Last Modified: | 18 May 2020 05:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18873 |
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