Reichert, J. C. and Epari, D. R. and Wullschleger, M. E. and Berner, A. and Saifzadeh, S. and Noeth, U. and Dickinson, I. C. and Schuetz, M. A. and Hutmacher, W. (2012) Bone tissue engineering. Reconstruction of critical sized segmental bone defects in the ovine tibia. ORTHOPADE, 41 (4). pp. 280-287. ISSN 0085-4530, 1433-0431
Full text not available from this repository. (Request a copy)Abstract
Well-established therapies for bone defects are restricted to bone grafts which face significant disadvantages (limited availability, donor site morbidity, insufficient integration). Therefore, the objective was to develop an alternative approach investigating the regenerative potential of medical grade polycaprolactone-tricalcium phosphate (mPCL-TCP) and silk-hydroxyapatite (silk-HA) scaffolds. Critical sized ovine tibial defects were created and stabilized. Defects were left untreated, reconstructed with autologous bone grafts (ABG) and mPCL-TCP or silk-HA scaffolds. Animals were observed for 12 weeks. Xray analysis, torsion testing and quantitative computed tomography (CT) analyses were performed. Radiological analysis confirmed the critical nature of the defects. Full defect bridging occurred in the autograft and partial bridging in the mPCL-TCP group. Only little bone formation was observed with silk-HA scaffolds. Biomechanical testing revealed a higher torsional moment/stiffness (p < 0.05) and CT analysis a significantly higher amount of bone formation for the ABG group when compared to the silk-HA group. No significant difference was determined between the ABG and mPCL-TCP groups. The results of this study suggest that mPCL-TCP scaffolds combined can serve as an alternative to autologous bone grafting in long bone defect regeneration. The combination of mPCL-TCP with osteogenic cells or growth factors represents an attractive means to further enhance bone formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; HUMAN OSTEOGENIC PROTEIN-1; MARROW STROMAL CELLS; IN-VITRO; COMPOSITE SCAFFOLDS; GRAFT SUBSTITUTES; AUTOLOGOUS BONE; SILK SCAFFOLDS; DIFFERENTIATION; HYDROXYAPATITE; Scaffold; Silk; Polycaprolactone; Calcium phosphate; Hydroxyapatite |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 May 2020 05:20 |
| Last Modified: | 15 May 2020 05:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18911 |
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