Ladwig, Friederike and Stahl, Mark and Ludewig, Uwe and Hirner, Axel A. and Hammes, Ulrich Z. and Stadler, Ruth and Harter, Klaus and Koch, Wolfgang (2012) Siliques Are Red1 from Arabidopsis Acts as a Bidirectional Amino Acid Transporter That Is Crucial for the Amino Acid Homeostasis of Siliques. PLANT PHYSIOLOGY, 158 (4). pp. 1643-1655. ISSN 0032-0889,
Full text not available from this repository. (Request a copy)Abstract
Many membrane proteins are involved in the transport of nutrients in plants. While the import of amino acids into plant cells is, in principle, well understood, their export has been insufficiently described. Here, we present the identification and characterization of the membrane protein Siliques Are Red1 (SIAR1) from Arabidopsis (Arabidopsis thaliana) that is able to translocate amino acids bidirectionally into as well as out of the cell. Analyses in yeast and oocytes suggest a SIAR1-mediated export of amino acids. In Arabidopsis, SIAR1 localizes to the plasma membrane and is expressed in the vascular tissue, in the pericycle, in stamen, and in the chalazal seed coat of ovules and developing seeds. Mutant alleles of SIAR1 accumulate anthocyanins as a symptom of reduced amino acid content in the early stages of silique development. Our data demonstrate that the SIAR1-mediated export of amino acids plays an important role in organic nitrogen allocation and particularly in amino acid homeostasis in developing siliques.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GREEN FLUORESCENT PROTEIN; CELL-TO-CELL; SEED DEVELOPMENT; GENE-EXPRESSION; SACCHAROMYCES-CEREVISIAE; PHLOEM TRANSPORT; THALIANA; PLANTS; IDENTIFICATION; NITROGEN; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 May 2020 07:08 |
| Last Modified: | 18 May 2020 07:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18963 |
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