Kistenpfennig, Christa and Hirsh, Jay and Yoshii, Taishi and Helfrich-Foerster, Charlotte (2012) Phase-Shifting the Fruit Fly Clock without Cryptochrome. JOURNAL OF BIOLOGICAL RHYTHMS, 27 (2). pp. 117-125. ISSN 0748-7304, 1552-4531
Full text not available from this repository. (Request a copy)Abstract
The blue light photopigment cryptochrome (CRY) is thought to be the main circadian photoreceptor of Drosophila melanogaster. Nevertheless, entrainment to light-dark cycles is possible without functional CRY. Here, we monitored phase response curves of cry(01) mutants and control flies to 1-hour 1000-lux light pulses. We found that cry(01) mutants phase-shift their activity rhythm in the subjective early morning and late evening, although with reduced magnitude. This phase-shifting capability is sufficient for the slowed entrainment of the mutants, indicating that the eyes contribute to the clock's light sensitivity around dawn and dusk. With longer light pulses (3 hours and 6 hours), wild-type flies show greatly enhanced magnitude of phase shift, but CRY-less flies seem impaired in the ability to integrate duration of the light pulse in a wild-type manner: Only 6-hour light pulses at circadian time 21 significantly increased the magnitude of phase advances in cry(01) mutants. At circadian time 15, the mutants exhibited phase advances instead of the expected delays. These complex results are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LOCOMOTOR-ACTIVITY RHYTHM; DROSOPHILA CLOCK; CIRCADIAN OSCILLATOR; COMPOUND EYES; LIGHT; NEURONS; ENTRAINMENT; PATHWAY; MUTANT; PHOTOSENSITIVITY; cryptochrome; light pulses; locomotor activity; Drosophila melanogaster |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 May 2020 07:24 |
| Last Modified: | 15 May 2020 07:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/18981 |
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