Reinhold, Stephan W. and Krueger, Bernd and Barner, Caroline and Zoicas, Flavius and Kammerl, Martin C. and Hoffmann, Ute and Bergler, Tobias and Banas, Bernhard and Kraemer, Bernhard K. (2012) Nephron-specific expression of components of the renin-angiotensin-aldosterone system in the mouse kidney. JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM, 13 (1). pp. 46-55. ISSN 1470-3203, 1752-8976
Full text not available from this repository. (Request a copy)Abstract
Introduction: The renin-angiotensin-aldosterone system (RAAS) plays an integral role in the regulation of blood pressure, electrolyte and fluid homeostasis in mammals. The capability of the different nephron segments to form components of the RAAS is only partially known. This study therefore aimed to characterize the nephron-specific expression of RAAS components within the mouse kidney. Materials and methods: Defined nephron segments of adult C57B/16 mice were microdissected after collagenase digestion. The gene expression of renin, angiotensinogen (AGT), angiotensin-converting enzyme (ACE), angiotensin II receptors 1a (AT1a), 1b (AT1b), and 2 (AT2) was assessed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Renin mRNA was present in glomeruli, in proximal tubules, in distal convoluted tubules (DCT) and cortical collecting ducts (CCD). AGT mRNA was found in proximal tubules, descending thin limb of Henle's loop (dTL) and in the medullary part of the thick ascending limb (mTAL). ACE mRNA was not detectable in microdissected mouse nephron segments. AT1a, AT1b and AT2 mRNA was detected in glomeruli and proximal convoluted tubules. Conclusions: Our data demonstrate a nephron-specific distribution of RAAS components. All components of the local RAAS - except ACE - are present in proximal convoluted tubules, emphasizing their involvement in sodium and water handling.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AT(2) RECEPTOR PROTEIN; IN-SITU HYBRIDIZATION; RAT PROXIMAL TUBULE; II-RECEPTOR; CONVERTING-ENZYME; MESSENGER-RNA; GENE-EXPRESSION; TYPE-2 RECEPTOR; LOCALIZATION; AT(1); Angiotensinogen; angiotensin; glomerulus; microdissection; mRNA; receptor; renin; RT-PCR; tubulus |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 May 2020 04:56 |
| Last Modified: | 19 May 2020 04:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19152 |
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