Bergner, Stefan and Wegener, Joachim and Matysik, Frank-Michael (2012) Monitoring passive transport of redox mediators across a confluent cell monolayer with single-cell resolution by means of scanning electrochemical microscopy. ANALYTICAL METHODS, 4 (3). pp. 623-629. ISSN 1759-9660,
Full text not available from this repository. (Request a copy)Abstract
Scanning electrochemical microscopy (SECM) was used for time-resolved studies of passive transport of redox active substances across cell monolayers with sub-cellular resolution. Platinum disk ultramicroelectrodes (UMEs) with effective electrode radii in the sub mm range were operated in the constant-height mode. Monolayers of normal rat kidney (NRK) or Madin Darby canine kidney strain II (MDCK II) epithelial cells served as model cell lines and were grown to confluence on permeable membrane filters to investigate the transport of redox mediators across epithelial barriers. A novel electrochemical cell was developed ensuring reliable mechanical fixation of the growth substrate and preventing membrane vibrations during the SECM operation. The detection of different redox mediators was enabled by potential switching at the UME. This concept proved to be an attractive alternative to the use of dual-microelectrodes or micropipette-microelectrode combinations. The various cell lines were investigated regarding their barrier functions with respect to different redox mediators. Para-and/or trans-cellular transport mechanisms could be distinguished using either hydrophilic ruthenium hexamine chloride or lipophilic ferrocene methanol as redox mediators.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLOOD-BRAIN-BARRIER; PERMEABILITY; MEMBRANE; DIFFUSION; SYSTEMS; SECM; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 May 2020 05:04 |
| Last Modified: | 19 May 2020 05:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19170 |
Actions (login required)
 |
View Item |
