Mayer, Florian and Kueper, Ulf and Meyer, Carolin and Daxer, Stefanie and Mueller, Volker and Rachel, Reinhard and Huber, Harald (2012) AMP-Forming Acetyl Coenzyme A Synthetase in the Outermost Membrane of the Hyperthermophilic Crenarchaeon Ignicoccus hospitalis. JOURNAL OF BACTERIOLOGY, 194 (6). pp. 1572-1581. ISSN 0021-9193,
Full text not available from this repository. (Request a copy)Abstract
Ignicoccus hospitalis, a hyperthermophilic, chemolithoautotrophic crenarchaeon was found to possess a new CO2 fixation pathway, the dicarboxylate/4-hydroxybutyrate cycle. The primary acceptor molecule for this pathway is acetyl coenzyme A (acetylCoA), which is regenerated in the cycle via the characteristic intermediate 4-hydroxybutyrate. In the presence of acetate, acetylCoA can alternatively be formed in a one-step mechanism via an AMP-forming acetyl-CoA synthetase (ACS). This enzyme was identified after membrane preparation by two-dimensional native PAGE/SDS-PAGE, followed by matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry and N-terminal sequencing. The ACS of I. hospitalis exhibits a molecular mass of similar to 690 kDa with a monomeric molecular mass of 77 kDa. Activity tests on isolated membranes and bioinformatic analyses indicated that the ACS is a constitutive membrane-associated (but not an integral) protein complex. Unexpectedly, immunolabeling on cells of I. hospitalis and other described Ignicoccus species revealed that the ACS is localized at the outermost membrane. This perfectly coincides with recent results that the ATP synthase and the H-2: sulfur oxidoreductase complexes are also located in the outermost membrane of I. hospitalis. These results imply that the intermembrane compartment of I. hospitalis is not only the site of ATP synthesis but may also be involved in the primary steps of CO2 fixation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARCHAEON HALOARCULA-MARISMORTUI; COA SYNTHETASE; A SYNTHETASE; NANOARCHAEUM-EQUITANS; PYROCOCCUS-FURIOSUS; ACETATE FORMATION; ESCHERICHIA-COLI; ATP SYNTHESIS; ASSOCIATION EQUILIBRIUM; METHANOTHRIX-SOEHNGENII; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 May 2020 05:18 |
| Last Modified: | 19 May 2020 05:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19175 |
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