Grosse, Jirka and Wehland, Markus and Pietsch, Jessica and Ma, Xiao and Ulbrich, Claudia and Schulz, Herbert and Saar, Katrin and Huebner, Norbert and Hauslage, Jens and Hemmersbach, Ruth and Braun, Markus and van Loon, Jack and Vagt, Nicole and Infanger, Manfred and Eilles, Christoph and Egli, Marcel and Richter, Peter and Baltz, Theo and Einspanier, Ralf and Sharbati, Soroush and Grimm, Daniela (2012) Short-term weightlessness produced by parabolic flight maneuvers altered gene expression patterns in human endothelial cells. FASEB JOURNAL, 26 (2). pp. 639-655. ISSN 0892-6638,
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This study focused on the effects of short-term microgravity (22 s) on the gene expression and morphology of endothelial cells (ECs) and evaluated gravisensitive signaling elements. ECs were investigated during four German Space Agency (Deutsches Zentrum fur Luft-und Raumfahrt) parabolic flight campaigns. Hoechst 33342 and acridine orange/ethidium bromide staining showed no signs of cell death in ECs after 31 parabolas (P31). Gene array analysis revealed 320 significantly regulated genes after the first parabola (P1) and P31. COL4A5, COL8A1, ITGA6, ITGA10, and ITGB3 mRNAs were down-regulated after P1. EDN1 and TNFRSF12A mRNAs were up-regulated. ADAM19, CARD8, CD40, GSN, PRKCA (all down-regulated after P1), and PRKAA1 (AMPK alpha 1) mRNAs (up-regulated) provide a very early protective mechanism of cell survival induced by 22 s microgravity. The ABL2 gene was significantly up-regulated after P1 and P31, TUBB was slightly induced, but ACTA2 and VIM mRNAs were not changed. beta-Tubulin immunofluorescence revealed a cytoplasmic rearrangement. Vibration had no effect. Hypergravity reduced CARD8, NOS3, VASH1, SERPINH1 (all P1), CAV2, ADAM19, TNFRSF12A, CD40, and ITGA6 (P31) mRNAs. These data suggest that microgravity alters the gene expression patterns and the cytoskeleton of ECs very early. Several gravisensitive signaling elements, such as AMPK alpha 1 and integrins, are involved in the reaction of ECs to altered gravity.-Grosse, J., Wehland, M., Pietsch, J., Ma, X., Ulbrich, C., Schulz, H., Saar, K., Hubner, N., Hauslage, J., Hemmersbach, R., Braun, M., van Loon, J., Vagt, N., Infanger, M., Eilles, C., Egli, M., Richter, P., Baltz, T., Einspanier, R., Sharbati, S., Grimm, D. Short-term weightlessness produced by parabolic flight maneuvers altered gene expression patterns in human endothelial cells. FASEB J. 26, 639-655 (2012). www.fasebj.org
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LEFT-VENTRICULAR HYPERTROPHY; FIBROBLAST-GROWTH-FACTOR; NF-KAPPA-B; SIMULATED MICROGRAVITY; F-ACTIN; ADHESION MOLECULE-1; MAMMALIAN-CELLS; GELSOLIN; APOPTOSIS; GRAVITY; angiogenesis; cytoskeleton; integrins; microgravity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 May 2020 04:54 |
| Last Modified: | 20 May 2020 04:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19296 |
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