Voelz, Ronny and von Lyncker, Ludwig and Baumann, Nadine and Dresselhaus, Thomas and Sprunck, Stefanie and Gross-Hardt, Rita (2012) LACHESIS-dependent egg-cell signaling regulates the development of female gametophytic cells. DEVELOPMENT, 139 (3). pp. 498-502. ISSN 0950-1991, 1477-9129
Full text not available from this repository. (Request a copy)Abstract
In contrast to animals, plant germ cells are formed along with accessory cells in specialized haploid generations, termed gametophytes. The female gametophyte of flowering plants consists of four different cell types, which exert distinct functions in the reproductive process. For successful fertilization, the development of the four cell types has to be tightly coordinated; however, the underlying mechanisms are not yet understood. We have previously isolated the lachesis (lis) mutant, which forms supernumerary gametes at the expense of adjacent accessory cells. LIS codes for the Arabidopsis homolog of the pre-mRNA splicing factor PRP4 and shows a dynamic expression pattern in the maturing female gametophyte. Here, we used LIS as a molecular tool to study cell-cell communication in the female gametophyte. We show that reducing LIS transcript amounts specifically in the egg cell, affects the development of all female gametophytic cells, indicating that cell differentiation in the female gametophyte is orchestrated by the egg cell. Among the defects observed is the failure of homotypic nuclei fusion in the central cell and, as a consequence, a block in endosperm formation. LIS-mediated egg cell signaling, thus, provides a safeguard mechanism that prevents the formation of nurturing tissue in the absence of a functional egg cell.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARABIDOPSIS-THALIANA; FERTILIZATION; COMMUNICATION; EMBRYO; PLANTS; FATE; Cell-cell communication; Egg cell; Female gametophyte |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 May 2020 08:29 |
| Last Modified: | 20 May 2020 08:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19344 |
Actions (login required)
 |
View Item |
