Penzkofer, A. and Stierl, M. and Hegemann, P. and Kateriya, S. (2012) Absorption and fluorescence characteristics of photo-activated adenylate cyclase nano-clusters from the amoeboflagellate Naegleria gruberi NEG-M strain. CHEMICAL PHYSICS, 392 (1). pp. 46-54. ISSN 0301-0104, 1873-4421
Full text not available from this repository. (Request a copy)Abstract
The spectroscopic characteristics of BLUF (BLUF = sensor of blue light using flavin) domain containing soluble adenylate cyclase (nPAC = Naegleria photo-activated cyclase) samples from the amoeboflagellate Naegleria gruberi NEG-M strain is studied at room temperature. The absorption and fluorescence spectroscopic development in the dark was investigated over two weeks. Attenuation coefficient spectra, fluorescence quantum distributions, fluorescence quantum yields, and fluorescence excitation distributions were measured. Thawing of frozen nPAC samples gave solutions with varying protein nano-cluster size and varying flavin, tyrosine, tryptophan, and protein color-center emission. Protein color-center emission was observed in the wavelength range of 360-900 nm with narrow emission bands of small Stokes shift and broad emission bands of large Stokes shift. The emission spectra evolved in time with protein nanocluster aging. (C) 2011 Elsevier B. V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SPECTROSCOPIC CHARACTERIZATION; COLOR-CENTERS; PROTEIN; DITYROSINE; CULTIVATION; SCATTERING; EVOLUTION; EMISSION; RESILIN; LASERS; Photo-activated adenylate cyclase; BLUF domain; Amoeboflagellate Naegleria gruberi; Flavin; Tyrosine; Tryptophan; Color center emission; Protein aging; Fluorescence quantum distributions; Fluorescence excitation spectra; Fluorescence quantum yields |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 May 2020 13:27 |
| Last Modified: | 20 May 2020 13:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19379 |
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