Ruedel, A. and Bosserhoff, A. K. (2012) Transfection Methods. In: UNSPECIFIED Methods in Cell Biology,, 112 . ELSEVIER ACADEMIC PRESS INC, SAN DIEGO, pp. 163-182. ISBN 978-0-12-405914-6
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It is an important method in molecular biology to up- or downregulate gene expression in cells. By transfection with expression constructs or silencing RNAs, the functionon genes can be defined and cellular reactions can be analyzed. For this purpose several methods are known and used to date. This article illustrates the most common protocols to introduce DNA or RNA into cells, including lipofection and electroporation. In the case of lipofection the nucleic acid is encapsulated in liposomes which fuse with the cell membrane, thereby releasing the nucleic acid into the cell. Cell division is required for DNA to get into the nucleus to be transcribed. In contrast to that, electroporation utilizes electrical pulses to create small pores in the cell membrane. This method allows transfection directly into the nucleus and therefore does not require cell division. Taken together, all methods can be used for cell transfection but it always has to be tested experimentally which method works best for each cell type.
| Item Type: | Book Section |
|---|---|
| Uncontrolled Keywords: | ; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jul 2020 09:39 |
| Last Modified: | 22 Jul 2020 09:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19394 |
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