Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

Lobasso, Simona and Lopalco, Patrizia and Angelini, Roberto and Vitale, Rita and Huber, Harald and Mueller, Volker and Corcelli, Angela (2012) Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus. ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL: 957852. ISSN 1472-3646, 1472-3654

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Abstract

The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80-90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N-acetylglucosamine- diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.

Item Type: Article
Uncontrolled Keywords: POLAR LIPIDS; HALOPHILIC ARCHAEON; HYDROTHERMAL VENT; PURPLE MEMBRANE; SP-NOV; ARCHAEBACTERIUM; CARDIOLIPIN; SULFUR; OXIDOREDUCTASE; BIOSYNTHESIS;
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum)
Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Dina Grohmann
Depositing User: Dr. Gernot Deinzer
Date Deposited: 22 May 2020 08:33
Last Modified: 22 May 2020 08:33
URI: https://pred.uni-regensburg.de/id/eprint/19416

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