Schulz, Christian and Stoelcker, Benjamin and Ruhland, Brigitte and Jentsch, Nico and Steege, Andreas (2012) Bronchoepithelial Expression of CXCR1 and CXCR2 Does Not Facilitate Transepithelial Migration of Neutrophils. RESPIRATION, 84 (2). pp. 108-116. ISSN 0025-7931,
Full text not available from this repository. (Request a copy)Abstract
Background: Neutrophilic airway inflammation is one of the key features of chronic obstructive pulmonary disease (COPD). The chemokine receptors 1 (CXCR1) and 2 (CXCR2) are expressed in the bronchial mucosa during chronic inflammation and might be of importance for transepithelial migration of neutrophils. Objectives: This study addressed the role of bronchoepithelial CXCR1 and CXCR2 expression with respect to transepithelial migration of neutrophils. Methods: Primary bronchial epithelial cells (PBECs) derived from COPD patients and healthy controls as well as transiently CXCR1- and CXCR2-transfected Calu-6 cells were used for transepithelial migration assays of neutrophils under various conditions. Epithelial CXCR1 and CXCR2 expression was verified by means of flow cytometry. Results: Transepithelial migration of neutrophils was significantly increased following lipopolysaccharide pretreatment of epithelial cells. Transient transfection of CXCR1 and CXCR2 neither augmented the transepithelial migration of neutrophils, nor did the selective blockade of CXCR1 and CXCR2 have any significant effect on neutrophilic transepithelial migration. In addition, no differences were found in PBECs and neutrophils derived from healthy controls and COPD patients. Conclusions: The data of the present study do not support the hypothesis that bronchoepithelial expression of CXCR1 and/or CXCR2 facilitate transepithelial migration of neutrophils. Copyright (C) 2011 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OBSTRUCTIVE PULMONARY-DISEASE; BRONCHIAL EPITHELIAL-CELLS; BRONCHOALVEOLAR LAVAGE; AIRWAY INFLAMMATION; INDUCED SPUTUM; INTERLEUKIN-8; RECEPTOR; COPD; SMOKERS; ANTAGONIST; Airway inflammation; Chemokine receptors; Chronic obstructive pulmonary disease; Epithelial cells; Inflammation; Neutrophils; Transepithelial migration |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 May 2020 10:55 |
| Last Modified: | 25 May 2020 10:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19453 |
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