Riechers, Alexander and Bosserhoff, Anja Katrin (2012) Pitfalls in immunohistochemistry - a recent example. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 5 (2). pp. 137-139. ISSN 1936-2625,
Full text not available from this repository. (Request a copy)Abstract
Immunohistochemistry is an important and valuable technique in many fields of research, although several common pitfalls can lead to wrong or misinterpreted results. A recently published study [1] claims that the protein MIA (melanoma inhibitory activity) is expressed in Purkinje cells in the cerebellum. Careful re-analysis resulted in negative results. Due to these results of our group we feel that this analysis could serve as example for the potential problems in immunohistochemistry caused by the combination of an unspecific antibody and the omission of evaluating control tissue samples.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MELANOMA INHIBITORY-ACTIVITY; RAP-DEFICIENT MICE; CARTILAGE; PROTEIN; EXPRESSION; CLONING; MIA; Immunohistochemistry; antibody; CD-RAP; MIA; specificity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 May 2020 08:43 |
| Last Modified: | 25 May 2020 08:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19506 |
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