Wu, Ming L. and van Teeseling, Muriel C. F. and Willems, Marieke J. R. and van Donselaar, Elly G. and Klingl, Andreas and Rachel, Reinhard and Geerts, Willie J. C. and Jetten, Mike S. M. and Strous, Marc and van Niftrik, Laura (2012) Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium. JOURNAL OF BACTERIOLOGY, 194 (2). pp. 284-291. ISSN 0021-9193,
Full text not available from this repository. (Request a copy)Abstract
Candidatus Methylomirabilis oxyfera is a newly discovered denitrifying methanotroph that is unrelated to previously known methanotrophs. This bacterium is a member of the NC10 phylum and couples methane oxidation to denitrification through a newly discovered intra-aerobic pathway. In the present study, we report the first ultrastructural study of "Ca. Methylomirabilis oxyfera" using scanning electron microscopy, transmission electron microscopy, and electron tomography in combination with different sample preparation methods. We observed that "Ca. Methylomirabilis oxyfera" cells possess an atypical polygonal shape that is distinct from other bacterial shapes described so far. Also, an additional layer was observed as the outermost sheath, which might represent a (glyco) protein surface layer. Further, intracytoplasmic membranes, which are a common feature among proteobacterial methanotrophs, were never observed under the current growth conditions. Our results indicate that "Ca. Methylomirabilis oxyfera" is ultrastructurally distinct from other bacteria by its atypical cell shape and from the classical proteobacterial methanotrophs by its apparent lack of intracytoplasmic membranes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METHANE-OXIDIZING BACTERIUM; ELECTRON-MICROSCOPY; S-LAYERS; METHYLOCOCCUS-CAPSULATUS; OXIDATION; CELL; VERRUCOMICROBIA; PHYLUM; ENRICHMENT; WATER; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 May 2020 10:40 |
| Last Modified: | 25 May 2020 10:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19610 |
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