Ackermann, M. and Kubitza, M. and Maier, K. and Brawanski, A. and Hauska, G. and Pina, A. L. (2011) THE VERTEBRATE HOMOLOG OF SULFIDE-QUINONE REDUCTASE IS EXPRESSED IN MITOCHONDRIA OF NEURONAL TISSUES. NEUROSCIENCE, 199. pp. 1-12. ISSN 0306-4522,
Full text not available from this repository. (Request a copy)Abstract
Hydrogen sulfide (H(2)S) can be consumed by both invertebrates and vertebrates as an inorganic substrate. The pathway metabolizing H(2)S probably involves three mitochondrial enzymes, one of which is sulfide-quinone oxidoreductase (SQR), known as sulfide-quinone reductase-like protein (SQRDL) in vertebrates. Evidence from fission yeast suggests that SQR might have a role in regulating sulfide levels in the cell. Regulation might be essential for H(2)S to act as a gaseous transmitter (gasotransmitter). The brain is an organ with high activity of gasotransmitters, like nitric oxide (NO) and H(2)S, which are known to affect synaptic transmission. In this study, we provide evidence that SQRDL is expressed in the mammalian brain. Real-time polymerase chain reaction (PCR) showed an increase in the number of Sqrdl transcripts in the brain with increasing age. Cellular fractionation and subsequent analysis by Western blotting indicated that the protein is located in mitochondria, which is the site of sulfide consumption in the cell. With an immunohistochemical approach, we demonstrated that the SQRDL protein is expressed in neurons, oligodendrocytes, and endothelial cells. Taken together, our data suggest that brain tissue harbors the machinery required for local regulation of sulfide levels. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NITRIC-OXIDE SYNTHASE; HYDROGEN-SULFIDE; ETHYLMALONIC ENCEPHALOPATHY; HUMAN-CELLS; OXIDOREDUCTASE; OXIDATION; GENE; INHIBITION; MECHANISM; SULFUR; sulfide-quinone reductase; SQRDL; hydrogen sulfide; mitochondria; neurons; oligodendrocytes |
| Subjects: | 500 Science > 580 Botanical sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurochirurgie Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Apr 2020 06:46 |
| Last Modified: | 01 Apr 2020 06:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19663 |
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