Wolff, Horst and Motyl, Magdalena and Hellerbrand, Claus and Heilmann, Joerg and Kraus, Birgit (2011) Xanthohumol Uptake and Intracellular Kinetics in Hepatocytes, Hepatic Stellate Cells, and Intestinal Cells. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59 (24). pp. 12893-12901. ISSN 0021-8561,
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Xanthohumol (XN) is the major prenylated chalcone of hops and hence an ingredient of beer. Despite many advances in understanding of the pharmacology of XN, one largely unresolved issue is its low bioavailability in the human organism. Also, not much is known about its actual concentrations and pharmacokinetics in liver and intestinal cells. Therefore, the uptake, intracellular distribution, and kinetics of XN were studied in various cell types, namely, hepatocellular carcinoma cells (HuH-7), hepatic stellate cells (HSC), primary cultured hepatocytes, and colorectal adenocarcinoma cells (Caco-2). Fluorescent microscopy allowed for the first time visualization and tracing of the uptake and intracellular distribution of XN. A rapid accumulation of XN concentrations that were up to >60-fold higher than the concentration present in the ambient culture medium was observed. Fluorescence recovery after photobleaching experiments revealed that most XN molecules are bound to cellular proteins, which may alter properties of cellular factors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LASER-SCANNING MICROSCOPE; HUMULUS-LUPULUS L.; SYSTEMATIC EVALUATION; HOPS; EXPRESSION; RECOVERY; MOBILITY; LINES; FRAP; TRANSPORTERS; FRAP; hepatic stellate cells; intracellular concentration; liver cells; xanthohumol |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 May 2020 12:26 |
| Last Modified: | 25 May 2020 12:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19665 |
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