Halder, Partho and Chen, Yi-chun and Brauckhoff, Janine and Hofbauer, Alois and Dabauvalle, Marie-Christine and Lewandrowski, Urs and Winkler, Christiane and Sickmann, Albert and Buchner, Erich (2011) Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain. PLOS ONE, 6 (12): e29352. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYACRYLAMIDE GELS; EH DOMAIN; PROTEIN; MELANOGASTER; THERAPEUTICS; GENE; BRUCHPILOT; HOMOLOGY; THERAPY; ASCITES; |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 12:38 |
| Last Modified: | 26 May 2020 12:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19678 |
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