Degen, Christian and Ecker, Josef and Piegholdt, Stefanie and Liebisch, Gerhard and Schmitz, Gerd and Jahreis, Gerhard (2011) Metabolic and growth inhibitory effects of conjugated fatty acids in the cell line HT-29 with special regard to the conversion of t11,t13-CLA. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1811 (12). pp. 1070-1080. ISSN 1388-1981,
Full text not available from this repository. (Request a copy)Abstract
Conjugated fatty acids (CFAs) exhibit growth inhibitory effects on colon cancer in vitro and in vivo. To investigate whether the anticancerogenic potency depends on number or configuration of the conjugated double bonds, the effect of conjugated linoleic acid (CLA; C18:2) isomers and conjugated linolenic acid (CLnA; C18:3) isomers on viability and growth of HT-29 cells were compared. Low concentrations of CLnAs (<10 mu m) yielded a higher degree of inhibitory effects compared to CLAs (40 mu M). All trans-CFAs were more effective compared to cis/trans-CFAs as follows: t9,t11,t13-CLnA >= c9,t11,t13-CLnA > t11,t13-CLA >= t9,t11-CLA > c9,t11-CLA. The mRNA expression analysis of important genes associated with fatty acid metabolism showed an absence of Delta 5-/Delta 6-desaturases and elongases in HT-29 cells, which was confirmed by fatty add analysis. Using time- and dose-dependent stimulation experiments several metabolites were determined. Low concentrations of all trans-CFAs (5-20 mu M) led to dose-dependent increase of conjugated t/t-C16:2 formed by beta-oxidation of C18 CFAs, ranging from 1-5% of total FAME. Importantly, it was found that CLnA is converted to CLA and that CLA is inter-converted (t11,t13-CLA is metabolized to c9,t11-CLA) by HT-29 cells. In summary, our study shows that growth inhibition of human cancer cells is associated with a specific cellular transcriptomic and metabolic profile of fatty acid metabolism, which might contribute to the diversified ability of CFAs as anti-cancer compounds. (C) 2011 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLON-CANCER CELLS; ALPHA-ELEOSTEARIC ACID; HUMAN TUMOR-CELLS; SEED OIL-RICH; LINOLEIC-ACID; LIPID-PEROXIDATION; LIQUID-CHROMATOGRAPHY; COLORECTAL-CANCER; DAIRY-PRODUCTS; LEUKEMIA-CELLS; Conjugated linoleic acid; Conjugated linolenic acid; Isomer specificity; Conversion; Colon cancer cell |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 05:39 |
| Last Modified: | 26 May 2020 05:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19705 |
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