Napp, Joanna and Behnke, Thomas and Fischer, Lorenz and Wuerth, Christian and Wottawa, Marieke and Katschinski, Doerthe M. and Alves, Frauke and Resch-Genger, Ute and Schaeferling, Michael (2011) Targeted Luminescent Near-Infrared Polymer-Nanoprobes for In Vivo Imaging of Tumor Hypoxia. ANALYTICAL CHEMISTRY, 83 (23). pp. 9039-9046. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
Polystyrene nanoparticles (PS-NPs) were doped with an oxygen-sensitive near-infrared (NIR)-emissive palladium meso-tetraphenylporphyrin and an inert reference dye which are both excitable at 635 nm. The nanosensors were characterized with special emphasis on fundamental parameters such as absolute photoluminescence quantum yield and fluorescence lifetime. The PS-NPs were employed for ratiometric dual-wavelength and lifetime-based photoluminescent oxygen sensing. They were efficiently taken up by cultured murine alveolar macrophages, yielding a characteristic and reversible change in ratiometric response with decreasing oxygen concentration. This correlated with the cellular hypoxic status verified by analysis of hypoxia inducible factor-1 alpha (HIF-1 alpha) accumulation. In addition, the surface of PS-NPs was functionalized with polyethylene glycol (PEG) and the monoclonal antibody herceptin, and their binding to HER2/neu-overexpressing tumor cells was confirmed in vitro. First experiments with tumor-bearing mouse revealed a distinctive ratiometric response within the tumor upon hypoxic condition induced by animal sacrifice. These results demonstrate the potential of these referenced NIR nanosensors for in vitro and in vivo imaging that present a new generation of optical probes for oncology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CALCIUM-PHOSPHATE NANOPARTICLES; PHOSPHORESCENT PROBES; INTRACELLULAR OXYGEN; DISSOLVED-OXYGEN; LIVE CELLS; CANCER; NANOSENSORS; FLUOROMETRY; FLUOROPHORE; COMPLEXES; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 08:31 |
| Last Modified: | 26 May 2020 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19732 |
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