Bauer, Sabrina and Weiss, Thomas S. and Wiest, Reiner and Schacherer, Doris and Hellerbrand, Claus and Farkas, Stefan and Scherer, Marcus N. and Ritter, Mirko and Schmitz, Gerd and Schaeffler, Andreas and Buechler, Christa (2011) Soluble CD163 is not increased in visceral fat and steatotic liver and is even suppressed by free fatty acids in vitro. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 91 (3). pp. 733-739. ISSN 0014-4800, 1096-0945
Full text not available from this repository. (Request a copy)Abstract
Visceral fat differs from subcutaneous fat by higher local inflammation and increased release of IL-6 and free fatty acids (FFA) which contribute to hepatic steatosis. IL-6 has been shown to upregulate the monocyte/macrophage specific receptor CD163 whose soluble form, sCD163, is increased in inflammatory diseases. Here, it was analyzed whether CD163 and sCD163 are differentially expressed in the human fat depots and fatty liver. CD163 mRNA and protein were similarly expressed in paired samples of human visceral and subcutaneous fat, and comparable levels in portal venous and systemic venous blood of liver-healthy controls indicate that release of sCD163 from visceral adipose tissue was not increased. CD163 was also similarly expressed in steatotic liver when compared to non-steatotic tissues and sCD163 was almost equal in the respective sera. Concentrations of sCD163 were not affected when passing the liver excluding substantial hepatic removal/release of this protein. A high concentration of IL-6 upregulated CD163 protein while physiological doses had no effect. However, sCD163 was not increased by any of the IL-6 doses tested. FFA even modestly decreased CD163 and sCD163. The anti-inflammatory mediators fenofibrate, pioglitazone, and eicosapentaenoic acid (EPA) did not influence sCD163 levels while CD163 was reduced by EPA. These data suggest that in humans neither visceral fat nor fatty liver are major sources of 5CD163. (C) 2011 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SCAVENGER RECEPTOR CD163; HUMAN MONOCYTE CD163; INSULIN-RESISTANCE; UP-REGULATION; ADIPOSE-TISSUE; MACROPHAGES; ACTIVATION; EXPRESSION; DISEASE; PLASMA; Portal vein; Fatty liver; CD163; Monocyte |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Kinder- und Jugendmedizin Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 08:45 |
| Last Modified: | 26 May 2020 08:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19736 |
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