Ramm, Paul and Bettscheider, Marc and Beier, Dagmar and Kalbitzer, Hans Robert and Kremer, Werner and Bogdahn, Ulrich and Hau, Peter and Aigner, Ludwig and Beier, Christoph P. (2011) H-1-Nuclear Magnetic Resonance Spectroscopy of Glioblastoma Cancer Stem Cells. STEM CELLS AND DEVELOPMENT, 20 (12). pp. 2189-2195. ISSN 1547-3287,
Full text not available from this repository. (Request a copy)Abstract
The noninvasive tracking of glioblastoma cancer stem cells (CSCs) in vivo constitutes a prerequisite for the development of CSC-specific therapies. Therefore, as a pilot study to identify CSC biomarkers for clinical magnetic resonance spectroscopy, 10 CSC lines were investigated using high-resolution H-1-nuclear magnetic resonance (H-1-NMR) spectroscopy at 600 and 800MHz (14.4 and 18.8 T) under reproducible in vitro conditions. The spectra were analyzed using principal component analysis (PCA), and spectral regions of high variability were evaluated regarding correlations to stem cell-related properties (clonogenic index and CD133 positivity) and cell death. PCA revealed that duplicates of CSC lines clustered together suggesting a characteristic H-1-NMR pattern of each CSC line. PCA enabled discrimination between samples with high and low clonogenicity, that is, clustering according to one of the hallmarks of stemness in samples with high viability. High/moderate correlations to clonogenicity and CD133 were found in spectral regions with high variability. In contrast, the mobile lipid signal at 1.28 ppm correlated to cell death, but not to stemness, as published previously for neural progenitor cells. In conclusion, our exploratory study demonstrates the correlation of specific resonances within H-1-NMR spectra with stem cell properties and advocates the use of the 1.28 ppm resonance as biomarker for cell death also in CSCs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEURAL PROGENITOR CELLS; NMR-SPECTROSCOPY; HUMAN BRAIN; RAT-BRAIN; MACROMOLECULE RESONANCES; MALIGNANT GLIOMA; MOBILE LIPIDS; IN-VIVO; H-1 MRS; TUMOR; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 09:44 |
| Last Modified: | 26 May 2020 09:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19756 |
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