Zhu, Wentao and Stevens, Axel P. and Dettmer, Katja and Gottfried, Eva and Hoves, Sabine and Kreutz, Marina and Holler, Ernst and Canelas, Andre B. and Kema, Ido and Oefner, Peter J. (2011) Quantitative profiling of tryptophan metabolites in serum, urine, and cell culture supernatants by liquid chromatography-tandem mass spectrometry. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 401 (10). pp. 3249-3261. ISSN 1618-2642, 1618-2650
Full text not available from this repository. (Request a copy)Abstract
A sensitive, selective, and comprehensive method for the quantitative determination of tryptophan and 18 of its key metabolites in serum, urine, and cell culture supernatants was developed. The analytes were separated on a C18 silica column by reversed-phase liquid chromatography and detected by electrospray ionization tandem mass spectrometry in positive ion multiple reaction monitoring (MRM) mode, except for indoxyl sulfate which was measured in negative ion MRM mode in a separate run. The limits of detection and lower limits of quantification were in the range of 0.1-50 and 0.5-100 nM, respectively. Fully C-13 isotope-labeled and deuterated internal standards were used to achieve accurate quantification. The applicability of the method to analyze serum, urine, and cell culture supernatants was demonstrated by recovery experiments and the evaluation of matrix effects. Precision for the analysis of serum, urine, and cell culture supernatants ranged between 1.3% and 16.0%, 1.5% and 13.5%, and 1.0% and 17.4%, respectively. The method was applied to analyze changes in tryptophan metabolism in cell culture supernatants from IFN-gamma-treated monocytes and immature or mature dendritic cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SOLID-PHASE EXTRACTION; HUMAN PLASMA; SEROTONIN; ACID; KYNURENINE; MONOCYTES; Tryptophan metabolism; LC-MS/MS; Serum; Urine; Cell culture supernatant |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 11:32 |
| Last Modified: | 26 May 2020 11:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19765 |
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