Jauch, Dominik and Martin, Maria and Schiechl, Gabriela and Kesselring, Rebecca and Schlitt, Hans Juergen and Geissler, Edward K. and Fichtner-Feigl, Stefan (2011) Interleukin 21 controls tumour growth and tumour immunosurveillance in colitis-associated tumorigenesis in mice. GUT, 60 (12). pp. 1678-1686. ISSN 0017-5749,
Full text not available from this repository. (Request a copy)Abstract
Background and aims Colitis-associated tumorigenesis is a balance between proliferation of tumour cells and tumour immunosurveillance. The role of T-helper-cell-derived cytokines in tumour growth is not fully understood. In this study the authors investigated the influence of interleukin (IL) 21 on intestinal tumorigenesis. Methods Chronic colitis was induced in IL-21(-/-) and littermate control wild-type mice with three cycles of 1.5% dextran sulphate sodium (DSS) over 7 days followed by 7 days of drinking water. Mice received an azoxymethane injection on day 0 of DSS-colitis to induce tumorigenesis. Immunohistochemistry was performed on inflamed and tumour-bearing areas of colons. Cytokine expression of isolated colonic CD4 T cells was determined by ELISA. Cytotoxic capacity of isolated colonic CD8 T cells targeting tumour cells was evaluated by flow cytometry and quantitative cytotoxicity assay. Apoptosis of tumour cells was determined by TUNEL assay of colonic sections. Results Increasing expression of IL-21 was observed in chronic colitis, which showed functional importance, since IL-21 deficiency prevented chronic DSS-colitis development. Further, in the absence of IL-21, significantly fewer tumour nodules were detected, despite a similar extent of intestinal inflammation. In wild-type mice, 8.6 +/- 1.9 tumour nodules were found compared with 1.0 +/- 1.2 in IL-21-deficient mice. In tumour-bearing IL-21-deficient mice, intestinal inflammation was restored and partly dependent on interferon (IFN)-gamma, whereas the inflammation in wild-type mice showed high IL-17A concentrations. In these rare tumours in IL-21-deficient mice, tumour cell proliferation (Ki-67) was decreased, while cell apoptosis was increased, compared with wild-type mice. Increased IFN gamma expression in tumour-bearing IL-21-deficient mice led to increased tumour immunosurveillance mediated by cytotoxic CD8CD103 T cells targeting E-cadherin(+) colonic tumour cells and therefore limited tumour growth. Conclusion These results indicate that IL-21 orchestrates colitis-associated tumorigenesis, leading to the hypothesis that high IFN gamma and low IL-17A expression reduces tumour cell proliferation and increases tumour immunosurveillance.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; PERFORIN-DEFICIENT MICE; NF-KAPPA-B; INTERFERON-GAMMA; T-CELLS; COLORECTAL-CANCER; CROHNS-DISEASE; IFN-GAMMA; IL-21; DIFFERENTIATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 11:54 |
| Last Modified: | 26 May 2020 11:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19773 |
Actions (login required)
 |
View Item |
