Ertl, Claudia and Lukowski, Robert and Sigl, Katja and Schlossmann, Jens and Hofmann, Franz and Wegener, Joerg W. (2011) Kinetics of relaxation by cGMP/cGKI signaling in fundus smooth muscle. EUROPEAN JOURNAL OF PHARMACOLOGY, 670 (1). pp. 266-271. ISSN 0014-2999,
Full text not available from this repository. (Request a copy)Abstract
cGMP-dependent kinase I (cGKI) is a major mediator of smooth muscle relaxation and exists in two isoforms, alpha and beta. Both isoforms are supposed to mediate their effects via different intracellular signaling pathways. To verify this concept, the kinetics of relaxation mediated by either isoform was analyzed in gastric fundus smooth muscle from mice. Muscles from mice that express selectively the I alpha or I beta isoform of cGKI in smooth muscle (sm-cGKI alpha or sm-cGKI beta mice) were compared to muscles from conventional cGKI(-/-) mice. Fundus muscles were contracted by carbachol and then relaxed by 8-Br-cGMP or by electrical field stimulation (EFS). The time course of relaxation by 8-Br-cGMP was not different between muscles from sm-cGKI alpha and sm-cGKI beta mice. EFS induced a fast transient relaxation in muscles from sm-cGKI alpha and sm-cGKI beta mice that was blocked by the NO synthase inhibitor L-NAME. Recovery from this relaxation was about 4-times slower in muscles from sm-cGKI alpha mice than in muscles from sm-cGKI beta mice. The different kinetic of recovery from relaxation after EFS in sm-cGKI alpha and sm-cGKI beta mice suggests that different signaling pathways exist for each cGKI isoform in vivo in fundus muscles. (C) 2011 Published by Elsevier B.V.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT PROTEIN-KINASE; VASOACTIVE INTESTINAL POLYPEPTIDE; LIGHT-CHAIN PHOSPHORYLATION; NITRIC-OXIDE; CYCLIC-GMP; CGMP KINASE; GASTRIC FUNDUS; RAT AORTA; MYOSIN PHOSPHATASE; GUANYLYL CYCLASE; Fundus; Relaxation; NANC; Nitric oxide; cGMP |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann, formerly Prof. Seifert) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 12:59 |
| Last Modified: | 26 May 2020 12:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19811 |
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