Kibler, Karen V. and Gomez, Carmen E. and Perdiguero, Beatriz and Wong, Shukmei and Huynh, Trung and Holechek, Susan and Arndt, William and Jimenez, Victoria and Gonzalez-Sanz, Ruben and Denzler, Karen and Haddad, Elias K. and Wagner, Ralf and Sekaly, Rafick P. and Tartaglia, James and Pantaleo, Giuseppe and Jacobs, Bertram L. and Esteban, Mariano (2011) Improved NYVAC-Based Vaccine Vectors. PLOS ONE, 6 (11): e25674. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
While as yet there is no vaccine against HIV/AIDS, the results of the phase III Thai trial (RV144) have been encouraging and suggest that further improvements of the prime/boost vaccine combination of a poxvirus and protein are needed. With this aim, in this investigation we have generated derivatives of the candidate vaccinia virus vaccine vector NYVAC with potentially improved functions. This has been achieved by the re-incorporation into the virus genome of two host range genes, K1L and C7L, in conjunction with the removal of the immunomodulatory viral molecule B19, an antagonist of type I interferon action. These novel virus vectors, referred to as NYVAC-C-KC and NYVAC-C-KC-DB19R, have acquired relevant biological characteristics, giving higher levels of antigen expression in infected cells, replication-competency in human keratinocytes and dermal fibroblasts, activation of selective host cell signal transduction pathways, and limited virus spread in tissues. Importantly, these replication-competent viruses have been demonstrated to maintain a highly attenuated phenotype.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | T-CELL RESPONSES; CLADE-C; GENE-EXPRESSION; VIRUS; MVA; IMMUNOGENICITY; ALVAC; DNA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 May 2020 14:32 |
| Last Modified: | 26 May 2020 14:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19817 |
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