Liu, Wen and Schreck, Carlos and Coleman, Richard A. and Wade, James B. and Hernandez, Yubelka and Zavilowitz, Beth and Warth, Richard and Kleyman, Thomas R. and Satlin, Lisa M. (2011) Role of NKCC in BK channel-mediated net K+ secretion in the CCD. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 301 (5). F1088-F1097. ISSN 1931-857X, 1522-1466
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Liu W, Schreck C, Coleman RA, Wade JB, Hernandez Y, Zavilowitz B, Warth R, Kleyman TR, Satlin LM. Role of NKCC in BK channel-mediated net K+ secretion in the CCD. Am J Physiol Renal Physiol 301: F1088-F1097, 2011. First published August 3, 2011; doi:10.1152/ajprenal.00347.2011.-Apical SK/ROMK and BK channels mediate baseline and flow-induced K secretion (FIKS), respectively, in the cortical collecting duct (CCD). BK channels are detected in acid-base transporting intercalated (IC) and Na-absorbing principal (PC) cells. Although the density of BK channels is greater in IC than PC, Na-K-ATPase activity in IC is considered inadequate to sustain high rates of urinary K secretion. To test the hypothesis that basolateral NKCC in the CCD contributes to BK channel-mediated FIKS, we measured net K secretion (J(K)) and Na absorption (J(Na)) at slow (similar to 1) and fast (similar to 5 nl.min(-1).mm(-1)) flow rates in rabbit CCDs microperfused in vitro in the absence and presence of bumetanide, an inhibitor of NKCC, added to the bath. Bumetanide inhibited FIKS but not basal J(K), J(Na), or the flow-induced [Ca2+](i) transient necessary for BK channel activation. Addition of luminal iberiotoxin, a BK channel inhibitor, to bumetanide-treated CCDs did not further reduce J(K). Basolateral Cl removal reversibly inhibited FIKS but not basal J(K) or J(Na). Quantitative PCR performed on single CCD samples using NKCC1- and 18S-specific primers and probes and the TaqMan assay confirmed the presence of the transcript in this nephron segment. To identify the specific cell type to which basolateral NKCC is localized, we exploited the ability of NKCC to accept NH4+ at its K-binding site to monitor the rate of bumetanide-sensitive cytosolic acidification after NH4+ addition to the bath in CCDs loaded with the pH indicator dye BCECF. Both IC and PC were found to have a basolateral bumetanide-sensitive NH4+ entry step and NKCC1-specific antibodies labeled the basolateral surfaces of both cell types in CCDs. These results suggest that BK channel-mediated FIKS is dependent on a basolateral bumetanide-sensitive, Cl-dependent transport pathway, proposed to be NKCC1, in both IC and PC in the CCD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CORTICAL COLLECTING DUCT; NA+-K+-2CL(-) COTRANSPORTER NKCC1; BASOLATERAL PLASMA-MEMBRANES; STIMULATED ATPASE ACTIVITIES; INTERCALATED CELL SUBTYPES; DISTAL TUBULAR POTASSIUM; POSTNATAL MATURATION; APICAL MEMBRANE; CL COTRANSPORTERS; RAT; apical membrane voltage; tubular flow rates; bumetanide; intercalated cell; principal cell |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Richard Warth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 May 2020 05:47 |
| Last Modified: | 27 May 2020 05:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19829 |
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