Hanses, Frank and Kopp, Andrea and Bala, Margarita and Buechler, Christa and Falk, Werner and Salzberger, Bernd and Schaeffler, Andreas (2011) Intracellular Survival of Staphylococcus aureus in Adipocyte-Like Differentiated 3T3-L1 Cells Is Glucose Dependent and Alters Cytokine, Chemokine, and Adipokine Secretion. ENDOCRINOLOGY, 152 (11). pp. 4148-4157. ISSN 0013-7227,
Full text not available from this repository. (Request a copy)Abstract
Although obesity and type 2 diabetes mellitus are associated with Gram-positive infections and a worse clinical outcome, it is unknown whether adipocytes can be infected by Gram-positive bacteria. Adipocyte-like differentiated 3T3-L1 cells and Staphylococcus aureus were used for infection experiments under normoglycemic (100 mg/dl) and hyperglycemic (450 mg/dl) conditions in the presence/absence of insulin (1 mu M). Intracellular presence and survival of S. aureus was investigated quantitatively. Supernatant cytokines, chemokines, and adipokines were measured by ELISA. Lipid metabolism and cellular morphology of infected adipocytes were investigated by different techniques. The present study provides the proof of principle that adipocyte-like cells can be infected by S. aureus dose dependently for up to 5 d. Importantly, low bacterial inocula did not affect cell viability. Intracellular survival of S. aureus was glucose dependent but not insulin dependent, and insulin receptor expression and insulin receptor signaling were not altered. Infection increased macrophage chemoattractant protein-1, visfatin, and IL-6 secretion, whereas resistin and adiponectin were decreased. Infected adipocytes had higher intracellular triacylglycerol concentrations and larger lipid droplets because of a decreased lipolysis. Taken together, infection of adipocytes by S. aureus is glucose dependent, inhibits cellular lipolysis, and affects the secretion of immunomodulating adipokines differentially. Because cell viability is not affected during infection, adipose tissue might function as a host for chronic infection by bacteria-causing metabolic, proinflammatory, and prodiabetic disturbances. (Endocrinology 152: 4148-4157, 2011)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TOLL-LIKE RECEPTORS; ADIPOSE-TISSUE; INSULIN-RESISTANCE; LIPOTEICHOIC ACID; TNF-ALPHA; ADIPONECTIN; INFECTION; INFLAMMATION; ASSOCIATION; METABOLISM; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 May 2020 09:19 |
| Last Modified: | 27 May 2020 09:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19900 |
Actions (login required)
 |
View Item |
