Karasi, J. C. and Dziezuk, F. and Quennery, L. and Foerster, S. and Reischl, U. and Colucci, G. and Schoener, D. and Seguin-Devaux, C. and Schmit, J. C. (2011) High correlation between the Roche COBAS (R) AmpliPrep/COBAS (R) TaqMan (R) HIV-1, v2.0 and the Abbott m2000 RealTime HIV-1 assays for quantification of viral load in HIV-1 B and non-B subtypes. JOURNAL OF CLINICAL VIROLOGY, 52 (3). pp. 181-186. ISSN 1386-6532,
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Background: HIV-1 viral load assays are critical tools to monitor antiretroviral therapy efficacy in HIV-infected patients. Two assays based on real-time PCR are available, the Abbott Real-Time HIV-1 assay (Abbott assay) and the new Roche COBAS (R) AmpliPrep/COBAS (R) TaqMan (R) HIV-1 test, v. 2.0 (TaqMan (R) test v2.0). Objectives: We have compared the performance of the two assays in 546 clinical plasma specimens of group M strains from Luxembourg and Rwanda. Study design: Our analyses focused on subtype inclusivity and platforms accuracy for 328 low level viremia samples. Results: Strong agreement and linear correlation were observed between the two assays (R(2) =0.95) over a wide dynamic range. Bland-Altman analysis showed a mean difference of 0.04 log 10 indicating minimal overall viral load quantification differences between both platforms. One subtype C was severely under-quantified by TaqMan (R) test v2.0 for which sequence analysis revealed multiple mismatches between the viral sequence and the primer/probe regions. A non significant lower quantification of the Abbott assay was shown for subtype A1 with a mean log 10 difference of 0.24. For specimens under 200 cp/mL, the overall agreement was 90% at the cut-off of 50 cp/mL and 67% at assay's lower limit of detection of 20 and 40 cp/mL. 309 samples were retested by the COBAS (R) AMPLICOR (R) HIV-1 MONITOR Test, v. 1.5 and a lack of agreement between the three assays around their lower limit of quantification was revealed. Conclusions: Both real-time tests were closely comparable in the quantification of viral load specimens of ten HIV-1 subtypes and recombinant forms. (C) 2011 Elsevier B. V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VIRUS TYPE-1 RNA; PERFORMANCE EVALUATION; PCR ASSAY; AMPLICOR; PLASMA; VERSION; MONITOR; QUANTITATION; INFECTION; REGIMEN; HIV-1; Viral-load; Real-time PCR; Subtype; Low level viremia |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 May 2020 09:31 |
| Last Modified: | 27 May 2020 09:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19904 |
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