Martins, Joana Raquel and Faria, Diana and Kongsuphol, Patthara and Reisch, Barbara and Schreiber, Rainer and Kunzelmann, Karl (2011) Anoctamin 6 is an essential component of the outwardly rectifying chloride channel. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108 (44). pp. 18168-18172. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
Outwardly rectifying chloride channels (ORCC, ICOR) of intermediate single-channel conductance of around 50 pS, are ubiquitously expressed, but have remained a mystery since their description more than 25 y ago. These channels have been shown to be activated on membrane excision and depolarization of the membrane voltage and by cAMP in the presence of the cystic fibrosis transmembrane conductance regulator. We show that anoctamin 6 (Ano6), a member of the recently identified family of putative Cl- channels, is the crucial component of ORCC single-channel and whole-cell currents in airway epithelial cells and Jurkat T lymphocytes. Cystic fibrosis transmembrane conductance regulator augmented ORCC produced by Ano6 in A549 airway epithelial cells. Ano6 is activated during membrane depolarization or apoptosis of Jurkat T lymphocytes and epithelial cells, and is inhibited by 5-nitro-2-(3-phenylpropylamino) benzoic acid, 4,4'-diisothio-cyanostilbene-2,2'-disulfonic acid, or AO1. Ano6 belongs to the basic equipment of any cell type, including colonic surface epithelial cells. It forms the essential component of ORCC and seems to have a role for cell shrinkage and programmed cell death.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSMEMBRANE CONDUCTANCE REGULATOR; CYSTIC-FIBROSIS; CL-CHANNEL; AIRWAY CELLS; CFTR; EXPRESSION; TMEM16A; DEATH; LYMPHOCYTES; ACTIVATION; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 May 2020 10:33 |
| Last Modified: | 27 May 2020 10:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19920 |
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