Schraivogel, Daniel and Weinmann, Lasse and Beier, Dagmar and Tabatabai, Ghazaleh and Eichner, Alexander and Zhu, Jia Yun and Anton, Martina and Sixt, Michael and Weller, Michael and Beier, Christoph P. and Meister, Gunter (2011) CAMTA1 is a novel tumour suppressor regulated by miR-9/9*in glioblastoma stem cells. EMBO JOURNAL, 30 (20). pp. 4309-4322. ISSN 0261-4189,
Full text not available from this repository. (Request a copy)Abstract
Cancer stem cells or cancer initiating cells are believed to contribute to cancer recurrence after therapy. MicroRNAs (miRNAs) are short RNA molecules with fundamental roles in gene regulation. The role of miRNAs in cancer stem cells is only poorly understood. Here, we report miRNA expression profiles of glioblastoma stem cell-containing CD133(+) cell populations. We find that miR-9, miR-9* (referred to as miR-9/9*), miR-17 and miR-106b are highly abundant in CD133(+) cells. Furthermore, inhibition of miR-9/9* or miR-17 leads to reduced neurosphere formation and stimulates cell differentiation. Calmodulin-binding transcription activator 1 (CAMTA1) is a putative transcription factor, which induces the expression of the anti-proliferative cardiac hormone natriuretic peptide A (NPPA). We identify CAMTA1 as an miR-9/9* and miR-17 target. CAMTA1 expression leads to reduced neurosphere formation and tumour growth in nude mice, suggesting that CAMTA1 can function as tumour suppressor. Consistently, CAMTA1 and NPPA expression correlate with patient survival. Our findings could provide a basis for novel strategies of glioblastoma therapy. The EMBO Journal (2011) 30, 4309-4322. doi: 10.1038/emboj.2011.301; Published online 19 August 2011
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGH-GRADE GLIOMA; MICRORNA TARGETS; ARGONAUTE PROTEINS; PREDICT PROGNOSIS; GENE-EXPRESSION; DOWN-REGULATION; CANCER; IDENTIFICATION; COMPLEXES; RECEPTOR; cancer stem cells; gene silencing; glioblastoma; microRNAs; tumour suppressor |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Jun 2020 05:14 |
| Last Modified: | 02 Jun 2020 05:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19962 |
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