Saleh, Sayed M. and Ali, Reham and Hirsch, Thomas and Wolfbeis, Otto S. (2011) Detection of biotin-avidin affinity binding by exploiting a self-referenced system composed of upconverting luminescent nanoparticles and gold nanoparticles. JOURNAL OF NANOPARTICLE RESEARCH, 13 (10). pp. 4603-4611. ISSN 1388-0764, 1572-896X
Full text not available from this repository. (Request a copy)Abstract
We demonstrate an affinity system based on the interaction of two types of nanoparticles. The first consists of upconverting luminescent NaYF4:Yb,Er nanoparticles (UCNPs) with a size of 40-100 nm, absorbing light in the infrared and showing luminescence at 521, 543 and at 657 nm. The second consists of (red) gold nanoparticles (Au-NPs) with a size of about 50 nm and capable of absorbing the green luminescence of the UCNPs. By labeling the UCNPs with avidin and the AuNPs with biotin we have established a model system for a self referenced affinity system applicable to sensing in biological samples. In the presence of avidin-modified UCNPs, the biotinylated Au-NPs can be detected in the range from 12 to 250 mu g mL(-1) by ratioing the intensity of the red (analyte-independent) emission to that of the green (analyte-dependent) emission band. The nanoparticles were characterized in terms of size and composition using transmission electron microscopy, thermogravimetry, and FTIR spectroscopy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESONANCE ENERGY-TRANSFER; PHOTON UP-CONVERSION; FLUORESCENT NANOPARTICLES; HOMOGENEOUS IMMUNOASSAY; CARBON NANOTUBES; SIZE; DNA; IMMOBILIZATION; NANOTECHNOLOGY; BIOTECHNOLOGY; Gold; Upconversion luminescence; Nanoparticle; Affinity assay; Biotin; Avidin; Non-covalent binding |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 May 2020 09:03 |
| Last Modified: | 28 May 2020 09:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20042 |
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